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negative control  (BPS Bioscience)


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    Structured Review

    BPS Bioscience negative control
    Negative Control, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sbe+reporter+kits/SBE+Reporter+Kit/pm41664195-81-18-20
    Average 93 stars, based on 17 article reviews
    negative control - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Activity Assay:

    Article Title: EPHB2 promotes diet-induced MASH liver fibrosis
    Article Snippet: .. The SBE reporter kits (79806 and 79578, BPS Biosciences) were used to monitor the activity of the TGFβ/SMAD signaling pathway in human HSCs, following the manufacturer’s instruction. .. HSCs were seeded into a 24-well plate, cultured to 70- 80% confluency and transfected with EPHB2 Mission®-esiRNA (Sigma Aldrich, USA) delivered into the cells using the lipofectamine ® RNAiMAX (Invitrogen, USA) according to the manufacturer’s instruction.



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    sbe smad binding element assay  (BPS Bioscience)
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    BPS Bioscience sbe smad binding element assay
    Merlin interacts with Arkadia and SKOR2 in the <t>SMAD-dependent</t> pathways in the TGFβ signaling. ( A ) Western blots of Arkadia and SKOR2 following the Co-IP with the Merlin antibody. ( B ) Western blots of Merlin and SKOR2 following the Co-IP with the Arkadia antibody. ( C ) Similar Western blot pattern of Ubiquitin following the Co-IP with the Merlin antibody with the Western blots of SKOR2 in panel A and B. ( D ) Western blots in the cytoplasmic fraction. ( E ) Western blots in the nuclear fraction. F-H . Quantification of Arkadia, SKOR2, and pSMAD2/3 in the nuclear fraction with TGFβ activation. I . The <t>SBE</t> assay indicated significantly higher response to the TGFβ activation in NF2 WT -SCs comparing to its in NF2 L64P - derived SCs
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    Image Search Results


    Merlin interacts with Arkadia and SKOR2 in the SMAD-dependent pathways in the TGFβ signaling. ( A ) Western blots of Arkadia and SKOR2 following the Co-IP with the Merlin antibody. ( B ) Western blots of Merlin and SKOR2 following the Co-IP with the Arkadia antibody. ( C ) Similar Western blot pattern of Ubiquitin following the Co-IP with the Merlin antibody with the Western blots of SKOR2 in panel A and B. ( D ) Western blots in the cytoplasmic fraction. ( E ) Western blots in the nuclear fraction. F-H . Quantification of Arkadia, SKOR2, and pSMAD2/3 in the nuclear fraction with TGFβ activation. I . The SBE assay indicated significantly higher response to the TGFβ activation in NF2 WT -SCs comparing to its in NF2 L64P - derived SCs

    Journal: Stem Cell Research & Therapy

    Article Title: Interactions among Merlin, Arkadia, and SKOR2 mediate NF2-associated human Schwann cell proliferation

    doi: 10.1186/s13287-025-04281-x

    Figure Lengend Snippet: Merlin interacts with Arkadia and SKOR2 in the SMAD-dependent pathways in the TGFβ signaling. ( A ) Western blots of Arkadia and SKOR2 following the Co-IP with the Merlin antibody. ( B ) Western blots of Merlin and SKOR2 following the Co-IP with the Arkadia antibody. ( C ) Similar Western blot pattern of Ubiquitin following the Co-IP with the Merlin antibody with the Western blots of SKOR2 in panel A and B. ( D ) Western blots in the cytoplasmic fraction. ( E ) Western blots in the nuclear fraction. F-H . Quantification of Arkadia, SKOR2, and pSMAD2/3 in the nuclear fraction with TGFβ activation. I . The SBE assay indicated significantly higher response to the TGFβ activation in NF2 WT -SCs comparing to its in NF2 L64P - derived SCs

    Article Snippet: SBE (smad binding element) assay was performed using SBE reporter kit (BPS Bioscience) following the product general protocol.

    Techniques: Western Blot, Co-Immunoprecipitation Assay, Activation Assay, Derivative Assay

    Reduction of SKOR2 protein level decreases the cell proliferation in SCs. ( A ) The proposed model of interaction among Merlin, Arkadia, and SKOR2 in responding to the SMAD-dependent TGF-β signaling pathway. ( B ) Representative images of NF2 L64P SCs infected with lentivirus for 72 h. Control: empty vector. SKOR2_KO: vector carrying a shRNA fragment. ( C ) Representative Western blots. ( D ) Quantitative ratio of SKOR2/β-actin. ( E ) Significantly lower cell proliferation level in SKOR2_KO SCs than control SCs based on the BrdU ELISA assay. Scale bad = 100 μm. t-test: p < 0.05

    Journal: Stem Cell Research & Therapy

    Article Title: Interactions among Merlin, Arkadia, and SKOR2 mediate NF2-associated human Schwann cell proliferation

    doi: 10.1186/s13287-025-04281-x

    Figure Lengend Snippet: Reduction of SKOR2 protein level decreases the cell proliferation in SCs. ( A ) The proposed model of interaction among Merlin, Arkadia, and SKOR2 in responding to the SMAD-dependent TGF-β signaling pathway. ( B ) Representative images of NF2 L64P SCs infected with lentivirus for 72 h. Control: empty vector. SKOR2_KO: vector carrying a shRNA fragment. ( C ) Representative Western blots. ( D ) Quantitative ratio of SKOR2/β-actin. ( E ) Significantly lower cell proliferation level in SKOR2_KO SCs than control SCs based on the BrdU ELISA assay. Scale bad = 100 μm. t-test: p < 0.05

    Article Snippet: SBE (smad binding element) assay was performed using SBE reporter kit (BPS Bioscience) following the product general protocol.

    Techniques: Infection, Control, Plasmid Preparation, shRNA, Western Blot, Enzyme-linked Immunosorbent Assay